Bottom-up proteomics

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Bottom-up proteomics
Method of protein identification that uses proteolytic digestion before analysis by liquid chromatography and mass spectrometry. Proteins can be isolated by gel electrophoresis prior to digestion or, in shotgun proteomics, the protein mixture is digested and the resulting peptides are separated by liquid chromatography.
Related Term(s): top-down proteomics

T. Baer, J. C. Morrow, J. D. Shao, S. Olesik. J. Am. Chem. Soc. 110, 5633 (1988). ( )

From Definitions of Terms Relating to Mass Spectrometry (IUPAC Recommendations 2013); DOI: 10.1351/PAC-REC-06-04-06 © IUPAC 2013.

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