Label-free quantification

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DRAFT DEFINITION
Label-free quantification (LFQ)
Method of determining the concentration of proteins through a combination of protein digestion and LC/MS without relying on stable isotope labeling. Protein quantification is achieved by measuring the signal intensity of peptide ions corresponding to a protein or by counting and comparing the number of fragment mass spectra identifying peptides of a given protein.
Related Term(s):
Reference(s):

M. Bantscheff, M. Schirle, G. Sweetman, J. Rick, B. Kuster, Anal. Bioanal. Chem. 389 (2007) 1017.

This is an unofficial draft definition presented for information and comment.

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