Multiple reaction monitoring

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IUPAC RECOMMENDATIONS 2013

K. K. Murray, R. K. Boyd, M. N. Eberlin, G. J. Langley, L. Li and Y. Naito, Pure Appl. Chem., 2013, 85, 1515-1609, 10.1351/PAC-REC-06-04-06.

Multiple reaction monitoring (MRM)
Application of selected reaction monitoring to multiple product ions from one or more precursor ions.
Note: This term should not be confused with consecutive reaction monitoring, which involves the serial application of three or more stages of selected reaction monitoring.
Related Term(s):
Reference(s):

P. Roepstorff, J. Fohlman. Biomed. Mass Spectrom. 11, 601 (1984).

Definitions of Terms Relating to Mass Spectrometry (IUPAC Recommendations 2013); DOI: 10.1351/PAC-REC-06-04-06 © IUPAC 2013.


Index of Terms

 




IUPAC reaction monitoring terms

Term Acronym Definition Diagram Reference
Selected ion monitoring SIM Operation of a mass spectrometer in which the abundances of ions of one or more specific m/z values are recorded rather than the entire mass spectrum.       Gold Book
Selected reaction monitoring SRM Data acquired from one or more specific product ions corresponding to m/z selected precursor ions recorded via two or more stages of mass spectrometry.
Note 1: Selected reaction monitoring in multiple-stage mass spectrometry is known as consecutive reaction monitoring.
Note 2: Selected reaction monitoring applied to multiple product ions from one or more precursor ions is known as multiple reaction monitoring.
SRM.jpg
de Hoffmann. J. Mass Spectrom. 31, 129 (1996).
Consecutive reaction monitoring CRM Multiple-stage mass spectrometry experiment with three or more stages of m/z separation in which products of sequential fragmentation or bimolecular reactions are selected for detection.
CRM.jpg
Tomer, Guenat, Deterding. Anal. Chem. 60, 2232 (1988).
Multiple reaction monitoring MRM Application of selected reaction monitoring to multiple product ions from one or more precursor ions.
Note: This term should not be confused with consecutive reaction monitoring, which involves the serial application of three or more stages of selected reaction monitoring.
MRM-Single.jpg
Roepstorff, Fohlman. Biomed. Mass Spectrom. 11, 601 (1984).

External links

  • Zakett, D.; Flynn, R. G. A.; Cooks, R. G. Chlorine Isotope Effects in Mass Spectrometry by Multiple Reaction Monitoring. J. Phys. Chem. 1978, 82, 2359–2362; http://dx.doi.org/10.1021/j100511a002
  • Lange, V.; Picotti, P.; Domon, B.; Aebersold, R. Selected Reaction Monitoring for Quantitative Proteomics: a Tutorial. Mol Syst Biol 2008, 4; http://dx.doi.org/10.1038/msb.2008.61
  • Sherman, J.; McKay, M. J.; Ashman, K.; Molloy, M. P. How Specific Is My SRM?: the Issue of Precursor and Product Ion Redundancy. Proteomics 2009, 9, 1120–1123; http://dx.doi.org/10.1002/pmic.200800577
  • Elschenbroich, S.; Kislinger, T. Targeted Proteomics by Selected Reaction Monitoring Mass Spectrometry: Applications to Systems Biology and Biomarker Discovery. Mol. BioSyst. 2011, 7, 292–303; http://dx.doi.org/10.1039/c0mb00159g
  • Bereman, M. S.; MacLean, B.; Tomazela, D. M.; Liebler, D. C.; MacCoss, M. J. The Development of Selected Reaction Monitoring Methods for Targeted Proteomics via Empirical Refinement; Proteomics 2012, 12, 1134–1141.http://dx.doi.org/10.1002/pmic.201200042
  • Kinter, M.; Kinter, C. S. Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics; 2013; http://www.springer.com/978-1-4614-8665-7