Quantitation by concatenated tryptic peptides

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IUPAC RECOMMENDATIONS 2013
Quantitation by concatenated tryptic peptides (QCAT)
Method for absolute quantitation of a protein or proteins in a multiplexed fashion using isotope-labeled peptides as surrogate internal standards. The labeled peptides are produced by synthesizing a gene coding for all desired peptides, expressing this gene in E. coli culture containing appropriately labeled substrate, and digesting with trypsin the expressed protein composed of the desired concatenated peptides.
Related Term(s):
Reference(s):

R. J. Beynon, M. K. Doherty, J. M. Pratt, S. J. Gaskell. Nature Meth. 2, 587 (2005). (http://dx.doi.org/10.1038/nmeth774 )

From Definitions of Terms Relating to Mass Spectrometry (IUPAC Recommendations 2013); DOI: 10.1351/PAC-REC-06-04-06 © IUPAC 2013.

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