Quantitation by concatenated tryptic peptides

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Quantitation by concatenated tryptic peptides (QCAT)
Method for absolute quantitation of a protein or proteins in a multiplexed fashion using isotope-labeled peptides as surrogate internal standards. The labeled peptides are produced by synthesizing a gene coding for all desired peptides, expressing this gene in E. coli culture containing appropriately labeled substrate, and digesting with trypsin the expressed protein composed of the desired concatenated peptides.
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R. J. Beynon, M. K. Doherty, J. M. Pratt, S. J. Gaskell. Nature Meth. 2, 587 (2005). (http://dx.doi.org/10.1038/nmeth774 )

From Definitions of Terms Relating to Mass Spectrometry (IUPAC Recommendations 2013); DOI: 10.1351/PAC-REC-06-04-06 © IUPAC 2013.

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