Stable isotope standards and capture by anti-peptide antibodies

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IUPAC RECOMMENDATIONS 2013
Stable isotope standards and capture by anti-peptide antibodies (SISCAPA)
Method for absolute quantitation of a protein or proteins in a multiplexed fashion using isotope-labeled peptides as surrogate internal standards. The labeled peptides are produced by synthesizing a gene coding for each desired peptide, cell-free transcription, and translation of this gene in a medium containing 13C6-labeled lysine, and digesting with trypsin the expressed protein composed of the desired concatenated peptides. For low-abundance proteins the sensitivity is increased by selective enrichment of the target peptides by antibody capture.
Related Term(s):
Reference(s):

L. Anderson, C. L. Hunter. Mol. Cell Proteomics 5, 573 (2006).

From Definitions of Terms Relating to Mass Spectrometry (IUPAC Recommendations 2013); DOI: 10.1351/PAC-REC-06-04-06 © IUPAC 2013.

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