Label-free quantitation

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Label-free quantitation
Method for determining the concentration of proteins through a combination of protein digestion and liquid chromatography-mass spectrometry (LC-MS) without relying on stable isotope labeling. Protein quantification is achieved by measuring the signal intensity of peptide ions corresponding to a protein or by counting and comparing the number of fragment mass spectra identifying peptides of a given protein.
Related Term(s):

M. Bantscheff, M. Schirle, G. Sweetman, J. Rick, B. Kuster. Anal. Bioanal. Chem. 389, 1017 (2007). ( )

From Definitions of Terms Relating to Mass Spectrometry (IUPAC Recommendations 2013); DOI: 10.1351/PAC-REC-06-04-06 © IUPAC 2013.

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