Shotgun proteomics
From MS Terms
IUPAC RECOMMENDATIONS 2013 |
Shotgun proteomics |
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Protein identification using a combination of high-performance liquid chromatography and mass spectrometry in which the proteins in a mixture are digested, usually by a specific enzyme, and the resulting peptides are separated by liquid chromatography and identified by tandem mass spectrometry. |
Related Term(s): bottom-up proteomics |
Reference(s):
M. L. Fournier, J. M. Gilmore, S. A. Martin-Brown, M. P. Washburn. Chem. Rev. 107, 3654 (2007). (http://dx.doi.org/10.1021/cr068279a ) |
From Definitions of Terms Relating to Mass Spectrometry (IUPAC Recommendations 2013); DOI: 10.1351/PAC-REC-06-04-06 © IUPAC 2013. |