Template:MRM deprecation

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Confusion regarding multiple reaction monitoring deprecation

Please note that the term multiple reaction monitoring is not deprecated in the IUPAC "Standard definitions of terms relating to mass spectrometry" Pure Appl. Chem., 2013, 85, 1515. There is no small amount of confusion regarding this fact due in part to the seven years that elapsed between the posting of the unreviewed 2006 draft of the document (still linked as "provisional recommendations" on the IUPAC website) and the publication of the peer reviewed document in 2013. Comments from reviewers during the peer review process led to a revision of the definition to what is now indicated on the multiple reaction monitoring page of this wiki. In several publications between 2006 and 2012, the draft definition was cited (e.g. http://dx.doi.org/10.1038/msb.2008.61, http://dx.doi.org/10.1002/pmic.200800577, http://dx.doi.org/10.1039/c0mb00159g, http://dx.doi.org/10.1002/pmic.201200042), inadvertently leading to further confusion. Again, please note that the IUPAC recommendation for multiple reaction monitoring is the one indicated in http://dx.doi.org/10.1351/PAC-REC-06-04-06.

Summary of reaction monitoring definitions

Term Acronym Definition Reference
Selected reaction monitoring SRM Data acquired from one or more specific product ions corresponding to m/z selected precursor ions recorded via two or more stages of mass spectrometry.
Note 1: Selected reaction monitoring in multiple-stage mass spectrometry is known as consecutive reaction monitoring.
Note 2: Selected reaction monitoring applied to multiple product ions from one or more precursor ions is known as multiple reaction monitoring.
de Hoffmann. J. Mass Spectrom. 31, 129 (1996).
Consecutive reaction monitoring CRM Multiple-stage mass spectrometry experiment with three or more stages of m/z separation in which products of sequential fragmentation or bimolecular reactions are selected for detection. Tomer, Guenat, Deterding. Anal. Chem. 60, 2232 (1988).
Multiple reaction monitoring MRM Application of selected reaction monitoring to multiple product ions from one or more precursor ions.
Note: This term should not be confused with consecutive reaction monitoring, which involves the serial application of three or more stages of selected reaction monitoring.
Roepstorff, Fohlman. Biomed. Mass Spectrom. 11, 601 (1984).