Top-down proteomics

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IUPAC RECOMMENDATIONS 2013
Top-down proteomics
Method of protein identification that uses the m/z selection of intact proteins followed by fragmentation and m/z separation in a second stage of mass spectrometry. Mixtures of proteins must first be separated by liquid chromatography or other separation method prior to analysis by mass spectrometry.
Related Term(s):
Reference(s):

S. K. Sze, Y. Ge, H. Oh, F. W. McLafferty. Proc. Natl. Acad. Sci. USA 99, 1774 (2002). (http://dx.doi.org/10.1073/pnas.251691898 )

N. L. Kelleher. Anal. Chem. 76, 197A (2004). (http://dx.doi.org/10.1021/ac0415657 )

From Definitions of Terms Relating to Mass Spectrometry (IUPAC Recommendations 2013); DOI: 10.1351/PAC-REC-06-04-06 © IUPAC 2013.

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