Proteomics
From Murray Mass Spectrometry Group
Proteomics is the study of proteins expressed by a particular genome. The Murray Group has several collaborative projects in this area.
Contents |
Group Members
Kermit Murray
Lancia Darville
Research
Isolation of Antimicrobial Peptides from the American Alligator (Alligator mississippiensis)
Significant anecdotal evidence exists to suggest that alligators and crocodiles are resistant to microbial infection. These animals typically live in environments with large concentrations of pathogenic microbes, yet their wounds typically heal without infection. Alligator leukocytes contain cationic peptides that are believed to be responsible for the strong antimicrobial activity against bacteria, virus and fungi. To begin to understand the structure and function of the peptides within the alligator’s blood that contribute to the antimicrobial activity we use a proteomics approach. Some of the methods used include one and two-dimensional gel electrophoresis, reversed phase HPLC and nano electrospray tandem mass spectrometry for detection and peptide sequencing.
This research is in collaboration with Dr. Mark Merchant at McNeese State University.
Lancia Darville, Doctoral Graduate Student and Dr. Mark E. Merchant.
Publications
- Bhattacharya, S. H.; Gal, A. A.; Murray, K. K., Laser Capture Microdissection MALDI for Direct Analysis of Archival Tissue. J Proteome Res 2003, 2, 95.
Abstracts
- ASMS 2008: Isolation and Identification of Low Molecular Weight Proteins from the American Alligator (Alligator mississippiensis) using Gel Separation with Nano ESI-Q-TOF
