K.K. Murray, D.H. Russell, Liquid Sample Introduction for Matrix-Assisted Laser Desorption Ionization, Anal. Chem., 65 (1993) 2534-2537; http://10.1021/ac00066a023.


Aerosol MALDI apparatus at Texas A&M University

In this paper initial results are presented for a new liquid introduction method for a mass spectrometer based on the application of MALDI to aerosols. Details of the method will be presented in future publications. Aerosols are formed using a continuous pneumatic aerosol generator from an acidified methanol solution containing the matrix and analyte. The solvent is removed by passing the aerosol through a heated tube to form a collimated beam of dried particles. Ions are formed by irradiating the aerosol with pulsed UV radiation in the source region of a linear TOF MS. The advantages of the aerosol MALDI TOF method are (1) liquid flow capacity of 0.5 mL/min, (2) rapid acquisition of the entire mass spectrum, and (3) ionization of large peptides and proteins. In this paper, the aerosol MALDI mass spectra of gramicidin S and lysozyme (chicken egg white) are presented. Flow injection mass spectra of gramicidin S are presented to demonstrate the feasibility of coupling aerosol MALDI with HPLC.

Kermit Murray at Texas A&M University in 1993
Kermit Murray at Texas A&M University in 1993
AMALDI side view
Texas A&M Aerosol MALDI instrument side view
TAMU AMALDI Mass Spectrometer 1992
Aerosol MALDI instrument at Texas A&M University, showing the flight tube (right) and ion source (left).
AMALDI Instrument at Texas A&M
Aerosol MALDI Instrument at Texas A&M University, 1992.
TAMU Roots pump
Roots blower and backing pump at Texas A&M University, 1992