C.A. Seneviratne, S. Ghorai, K.K. Murray, “Laser desorption sample transfer for gas chromatography/mass spectrometry,” Rapid Commun. Mass Spectrom. 30 (2016) 89–94. doi:10.1002/rcm.7419.

Rationale: Ambient mass spectrometry can detect small molecules directly, but complex mixtures can be a challenge. We have developed a method that incorporates small molecule separation based on laser desorption with capture on a solid‐phase microextraction (SPME) fiber for injection into a gas chromatography/mass spectrometry (GC/MS) system.
Methods: Samples on a metal target were desorbed by a 3 µm mid‐infrared laser focused to a 250 µm spot and 1.2 mJ pulse energy. The desorbed material was aspirated into a metal tube suspended 1 mm above the laser spot and captured on a SPME fiber. The collected material was injected into a GC/MS instrument for analysis.
Results: We have developed a versatile approach for ambient laser desorption sampling onto SPME for GC/MS analysis. The performance of the laser desorption SPME capture GC/MS system was demonstrated for small molecule standards, a mixture of nitroaromatic explosives, and collected cigarette smoke.
Conclusions: The utility of ambient laser desorption sampling onto SPME for GC/MS was demonstrated. The performance of the method was evaluated by preparing calibration standards of caffeine over a range from 200 to 1000 ng. Laser desorption ambient sampling of complex mixtures was accomplished using SPME GC/MS.

Schematic representation of the experimental configuration for laser desorption sample transfer to SPME fiber. The heated transfer line is held 1 mm above the sample surface and the SPME fiber is inserted into a tee in the tube and exposed to the flow.