Deep-ultraviolet laser ablation sampling for proteomic analysis of tissue

R.O. Lawal, L.T. Richardson, C. Dong, F. Donnarumma, T. Solouki, K.K. Murray, Deep-ultraviolet laser ablation sampling for proteomic analysis of tissue, Anal. Chim. Acta, 1184 (2021). doi: 10.1016/j.aca.2021.339021 Abstract Deep-ultraviolet laser ablation with a pulsed 193 nm ArF excimer laser was used to remove localized regions from tissue sections from which proteins were extracted for […]

Multimodal Label‐Free Monitoring of Adipogenic Stem Cell Differentiation Using Endogenous Optical Biomarkers

N. Mehta, S. Shaik, A. Prasad, A. Chaichi, S.P. Sahu, Q. Liu, S.M.A. Hasan, E. Sheikh, F. Donnarumma, K.K. Murray, X. Fu, R. Devireddy, M.R. Gartia, Multimodal Label‐Free Monitoring of Adipogenic Stem Cell Differentiation Using Endogenous Optical Biomarkers, Adv. Funct. Mater., (2021) 2103955; doi: 10.1002/adfm.202103955 Abstract Stem cell-based therapies carry significant promise for treating human […]

Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer

A.C. Pulukkody, Y.P. Yung, F. Donnarumma, K.K. Murray, R.P. Carlson, L. Hanley, Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer, PLoS One, 16 (2021) e0250911, doi: 10.1371/journal.pone.0250911 Abstract Heterogeneity in the distribution of nutrients and oxygen gradients during biofilm growth gives rise to changes in phenotype. There has been […]

Lasers for matrix-assisted laser desorption ionization

K.K. Murray, Lasers for matrix-assisted laser desorption ionization, J. Mass Spectrom., 56 (2021) e4664. https://doi.org/10.1002/jms.4664 Abstract Matrix-assisted laser desorption ionization (MALDI) was introduced 35 years ago and has advanced from a general method for producing intact ions from large biomolecules to wide use in applications ranging from bacteria identification to tissue imaging. MALDI was enabled […]