Group News

IR Opolette 2940

IR Opolette 2940 side view.
IR Opolette 2940 side view.
Another laser and the second from OPOTEK this year: an IR Opolette 2940. It is fixed wavelength at 2940 nm for no other reason than that is the Er:YAG laser wavelength. It is possible to manually adjust the internal optics to generate light from 2700 to 3100 nm.

OPOTEK IR Opolette head (right) and Quantel
OPOTEK IR Opolette head (right) and Quantel

This laser has its roots in the STTR grant that I had with OPOTEK starting in 2001 (awarded when I was at Emory but moved immediately to LSU in the first year of Phase I). Our goal was to build an OPO with the capabilities of the Mirage 3000B but in a smaller package.

OPOTEK Opolette 2940 laser head.
OPOTEK Opolette 2940 laser head.

The left port gives access to the Nd:YAG laser fundamental at 1064 nm and the right port is the 2940 nm mid-IR at about 3 mJ per pulse at 20 Hz. Plenty of energy when focused to efficiently ablate thin films (similar to our wavelength tunable IR Opolette which you can see ablating things here.)

High Resolution Laser Mass Spectrometry Bioimaging

K. K. Murray, , C. A. Seneviratne, and S. Ghorai, “High Resolution Laser Mass Spectrometry Bioimaging” Methods 104 (2016) 118–126; doi:10.1016/j.ymeth.2016.03.002

Mass spectrometry imaging (MSI) was introduced more than five decades ago with secondary ion mass spectrometry (SIMS) and a decade later with laser desorption/ionization (LDI) mass spectrometry (MS). Large biomolecule imaging by matrix-assisted laser desorption/ionization (MALDI) was developed in the 1990s and ambient laser MS a decade ago. Although SIMS has been capable of imaging with a moderate mass range at sub-micrometer lateral resolution from its inception, laser MS requires additional effort to achieve a lateral resolution of 10 lm or below which is required to image at the size scale of single mammalian cells. This review covers untargeted large biomolecule MSI using lasers for desorption/ionization or laser desorption and post-ionization. These methods include laser microprobe (LDI) MSI, MALDI MSI, laser ambient and atmospheric pressure MSI, and near-field laser ablation MS. Novel approaches to improving lateral resolution are discussed, including oversampling, beam shaping, transmission geometry, reflective and through-hole objectives, microscope mode, and near-field optics.

http://mass-spec.lsu.edu/documents/murray_2016.pdf