Atomic force microscope stage with capture wire for tip enhanced laser ablation

Atomic Force Microscopy Tip Enhanced Laser Ablation

We are combining atomic force microscopy (AFM) with near-field tip-enhanced laser ablation for sub-micrometer sampling from cells and tissue. This approach has applications in single cell bioanalysis by mass spectrometry and genomic sequencing.

Atomic force microscope with pulsed laser ablation and sample capture
Atomic force microscope with pulsed laser ablation and sample capture

Ghorai, Seneviratne, Murray, “Tip-Enhanced Laser Ablation Sample Transfer for Biomolecule Mass Spectrometry.” J. Am. Soc. Mass Spectrom. 2015, 26, 63–70.

AFM image of ablation crater of an insulin thin film and MALDI mass spectrum of the collected material
AFM image of ablation crater of an insulin thin film and MALDI mass spectrum of the collected material

Murray, Ghorai, Seneviratne, “Tip Enhanced Laser Ablation Sample Transfer for Mass Spectrometry.” MRS Proc. 2015, 1754, mrsf14–1754–pp08–04.