The microfluidics project is aimed at developing an interface that allows microfluidic chip devices to be “read” by a mass spectrometer. Microfluidic chips are small devices with systems of micrometer sized channels etched into glass, silicon, or molded in plastic. The channels are enclosed by bonding to a flat substrate. Various microfluidic components have been developed to perform valving, mixing, filtering, electrophoresis, liquid chromatography, polymerase chain reaction and other procedures. Sample transport is typically by electroosmosis and/or electrophoresis. In analogy to electronic integrated circuits, multiple components can be combined to form microscale total analysis systems. The advantages of such devices compared to conventional chemical analysis include low cost, small size, low sample consumption, high sensitivity, and rapid analysis.
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- J. Lee, S. A. Soper and K. K. Murray, “Development of an efficient on-chip digestion system for protein analysis using MALDI-TOF MS,“ Analyst 134, 2426 (2009).
- Lee, S. A. Soper, and K. K. Murray “Microﬂuidic chips with MALDI analysis for proteomics – A review,” Anal. Chim. Acta. 649, 180 (2009).
- J. Lee, S. A. Soper, and K. K. Murray “Microﬂuidic chips for mass spectrometry-based proteomics,” J. Mass Spectrom. 44, 579 (2009).
- Lee, Musyimi, Soper, Murray, Development of an Automated Digestion and Droplet Deposition Microfluidic Chip for MALDI-TOF MS. J. Am. Soc. Mass Spectrom. 2008, 19, 964–972.