3/5/09 12:34 PM

MALDI Microfluidics

 

Rotating Ball Inlet

The rotating ball inlet is a continuous MALDI interface in which the ball transports the matrix and analyte deposit to the laser. The deposition can be done at atmospheric pressure with the ball acting as a vacuum seal.

Rotating ball inlet with capillary electrophoresis microfluidic chip.
Rotating ball inlet with capillary electrophoresis microfluidic chip.
Rotating ball inlet detail
Rotating ball inlet detail
  • Musyimi, Guy, Narcisse, Soper, Murray, Direct Coupling of Polymer-Based Microchip Electrophoresis to Online MALDI-MS Using a Rotating Ball Inlet. Electrophoresis 2005, 26, 4703–4710.
    A schematic diagram showing the top view of the chip to ball direct contact deposition: (A) sample reservoir, (B) waste reservoir, (C) buffer reservoir,  (D) fluidic channel exit tip, (E) (E) ball. Panels on the right show two scanning electron micros- copy (SEM) images of (1) the brass mold insert and (2) an embossed microchip tip.
    A schematic diagram showing the top view of the chip to ball direct contact deposition: (A) sample reservoir, (B) waste reservoir, (C) buffer reservoir, (D) fluidic channel exit tip, (E) (E) ball. Panels on the right show two scanning electron micros- copy (SEM) images of (1) the brass mold insert and (2) an embossed microchip tip.
  • Musyimi, Narcisse, Zhang, Stryjewski, Soper, Murray, Online CE-MALDI-TOF MS Using a Rotating Ball Interface. Anal. Chem. 2004, 76, 5968–5973.
    A schematic representation of the rotating ball inlet interface for CE-MS indicating: ball A, driveshaft B, teflon gasket C, ISO-100 flange D, extraction lens E, matrix capillary F, buffer capillary G, anode and running buffer H, electrophoresis capillary I, cleaning solvent capillary J, felt pad and holder K and solvent drain L.
    A schematic representation of the rotating ball inlet interface for CE-MS indicating: ball A, driveshaft B, teflon gasket C, ISO-100 flange D, extraction lens E, matrix capillary F, buffer capillary G, anode and running buffer H, electrophoresis capillary I, cleaning solvent capillary J, felt pad and holder K and solvent drain L.
    A two dimensional representation of a CE-MALDI separation of the peptides substance p, vasopressin and neurotensin. The gray scale intensity represents the ion signal at a particular m/z and elution time.
    A two dimensional representation of a CE-MALDI separation of the peptides substance p, vasopressin and neurotensin. The gray scale intensity represents the ion signal at a particular m/z and elution time.
  • Zhang, Narcisse, Murray, On-Line Single Droplet Deposition for MALDI Mass Spectrometry. J. Am. Soc. Mass Spectrom. 2004, 15, 1471–1477.
    Diagram of the single droplet deposition device: A, 19 mm diameter stainless steel ball; B, drive shaft; C, gasket; D, ISO 100 flange; E, ground grid; F, droplet generation electronics; G, syringe pump for matrix solution; H, syringe pump for solvent; I, capillary; J, translation stage for capillary; K, droplet generator; L, translation stage for droplet generator; M, cleaning system (Teflon holder and felt); N, peek plastic tube for waste; O, syringe pump for analyte solution.
    Diagram of the single droplet deposition device: A, 19 mm diameter stainless steel ball; B, drive shaft; C, gasket; D, ISO 100 flange; E, ground grid; F, droplet generation electronics; G, syringe pump for matrix solution; H, syringe pump for solvent; I, capillary; J, translation stage for capillary; K, droplet generator; L, translation stage for droplet generator; M, cleaning system (Teflon holder and felt); N, peek plastic tube for waste; O, syringe pump for analyte solution.
  • Orsnes, Graf, Degn, Murray, A Rotating Ball Inlet for on-Line MALDI Mass Spectrometry. Anal. Chem. 2000, 72, 251–254.
     Diagram of the vacuum version of the online ROBIN-MALDI probe. A, 10 mm in diameter stainless steel ball; B, drive shaft; C, gasket; D, adjustment screw; E, repeller; F, extraction grid; G, ground grid; H, capillary. The ball is rotated through the shaft, which is connected to a gear motor positioned outside the vacuum chamber.
    Diagram of the vacuum version of the online ROBIN-MALDI probe. A, 10 mm in diameter stainless steel ball; B, drive shaft; C, gasket; D, adjustment screw; E, repeller; F, extraction grid; G, ground grid; H, capillary. The ball is rotated through the shaft, which is connected to a gear motor positioned outside the vacuum chamber.