Deep-ultraviolet laser ablation sampling for proteomic analysis of tissue

R.O. Lawal, L.T. Richardson, C. Dong, F. Donnarumma, T. Solouki, K.K. Murray, Deep-ultraviolet laser ablation sampling for proteomic analysis of tissue, Anal. Chim. Acta, 1184 (2021). doi: 10.1016/j.aca.2021.339021 Abstract Deep-ultraviolet laser ablation with a pulsed 193 nm ArF excimer laser was used to remove localized regions from tissue sections from which proteins were extracted for […]

Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer

A.C. Pulukkody, Y.P. Yung, F. Donnarumma, K.K. Murray, R.P. Carlson, L. Hanley, Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer, PLoS One, 16 (2021) e0250911, doi: 10.1371/journal.pone.0250911 Abstract Heterogeneity in the distribution of nutrients and oxygen gradients during biofilm growth gives rise to changes in phenotype. There has been […]

MALDI imaging directed laser ablation tissue microsampling for data independent acquisition proteomics

K. Wang, F. Donnarumma, M.E. Pettit, C.W. Szot, T. Solouki, K.K. Murray, MALDI imaging directed laser ablation tissue microsampling for data independent acquisition proteomics, J. Mass Spectrom., 55 (2020) e4475; doi: 10.1002/jms.4475 Abstract A multimodal workflow for mass spectrometry imaging was developed that combines MALDI imaging with protein identification and quantification by liquid chromatography tandem […]

IMSC 2018: Infrared Laser Ablation Microsampling Coupled with MALDI Imaging

International Mass Spectrometry Conference, Florence, Italy, August 28, 2018 Louisiana State University: Kermit K. Murray, Fabrizio Donnarumma, Kelin Wang, Carson W. Szot, Chao Dong,Baylor University: Touradj Solouki, Michael E. Pettit Introduction Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is a powerful method for determining the location of biomolecules in tissue; however, protein identification and […]

IMSC 2018: Combined Infrared and Ultraviolet Ablation Electrospray Ionization Mass Spectrometry

International Mass Spectrometry Conference, Florence, Italy, August 27, 2018 Kermit K. Murray, Remi O. Lawal, and Fabrizio Donnarumma Louisiana State University, Baton Rouge, LA Introduction We are using combined mid-infrared and deep-ultraviolet two-laser ablation coupled with electrospray ionization for ambient mass spectrometry of biomolecules in tissue. The goal is to increase nanoparticle production and improve […]