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- The analysis of several batches of commercial biopharmaceutical product Dupixent using top-down intact mass spectrometry revealed that this immunoglobulin IgG4 features a small amount of O-link glycosylation in Fab region. This is the first report of an O-link glycosylation in IgG4 antibody. The paper describes most likely structure of the O-link glycosylation as well as probable location(s). The relative quantification showed only small quantity of the modification but appearing consistently in several batches of Dupixent. The O-link glycosylation site was […]
- Publication date: Available online 26 April 2024Source: Clinica Chimica ActaAuthor(s): Sylvia Karin Neef, Anna-Diana Hinderer, Wassim Arbash, Fritz Sörgel, Christian Wunder, Matthias Schwab, Ute Hofmann
- The transcription factor STE12 influences growth on several carbon sources and production of dehydroacetic acid (DHAA) in Trichoderma reesei
- Deep-time phylogenetic inference by paleoproteomic analysis of dental enamel
- Introduction Alzheimer's disease (AD) is the most prevalent neurodegenerative disease, yet our comprehension predominantly relies on studies within the non-Hispanic White (NHW) population. Here we aimed to provide comprehensive insights into the proteomic landscape of AD across diverse racial and ethnic groups. Methods Dorsolateral prefrontal cortex (DLPFC) and superior temporal gyrus (STG) brain tissues were donated from multiple centers (Mayo Clinic, Emory University, Rush University, Mt. Sinai School of Medicine) and were harmonized through neuropathological evaluation, specifically adhering to the […]
- Introduction: Co-fractionation mass spectrometry couples native-like separations of protein/protein complexes with mass spectrometric proteome analysis for global characterization of protein networks. The technique allows for both de novo detection of complexes and for the detection of subtle changes in their protein composition. The typical requirement for fine-grained fractionation of >80 fractions, however, translates into significant demands on sample quantity and mass spectrometric instrument time, and represents a significant barrier to experimental replication and the use of scarce sample material (ex. […]
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- Publication date: June 2024Source: International Journal of Mass Spectrometry, Volume 500Author(s):
- Publication date: June 2024Source: International Journal of Mass Spectrometry, Volume 500Author(s):
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.3c00431
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.4c00098
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.4c00076
- Mass Spectrometry Reviews, EarlyView.
- Chemistry – A European Journal, Accepted Article.
- Abstract Diquat (DQ), paraquat (PQ), glufosinate (GLU), and glyphosate (GLYP) are commonly used herbicides that have been confirmed to be toxic to humans. Rapid and accurate measurements of these toxicants in clinical practice are beneficial for the correct diagnosis and timely treatment of herbicide-poisoned patients. The present study aimed to establish an efficient, convenient, and reliable method to achieve the simultaneous quantification of DQ, PQ, GLU, and GLYP in human plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS) without using derivatization […]
- Urinary metabolomic profiling of a cohort of Colombian patients with systemic lupus erythematosus
- Pancreatic {beta}-cells in the islets of Langerhans are key to maintaining glucose homeostasis, by secreting the peptide hormone insulin. Insulin is packaged within vesicles named insulin secretory granules (ISGs), that have recently been considered to have intrinsic structures and proteins that regulate insulin granule maturation, trafficking, and secretion. Previously, studies have identified a handful of novel ISG-associated proteins using different separation techniques. Here, this study combines an optimized ISG isolation technique and mass spectrometry-based proteomics, with an unbiased protein correlation […]
- Pyro(phospho)mania
- Extensive protein pyrophosphorylation revealed in human cell lines
- Publication date: Available online 25 April 2024Source: Journal of Chromatography AAuthor(s): Fenfang Deng, Jia He, Yingyi Dai, Rongfei Peng, Xinhong Pan, Jun Yuan, Lei Tan
- Denaturing mass photometry for rapid optimization of chemical protein-protein cross-linking reactions
- Electrospray-assisted cryo-EM sample preparation to mitigate interfacial effects
- We developed a simple, paper-based device that enables sensitive detection by mass spectrometry (MS) without solid phase extraction or other sample preparation. Using glass fiber filter papers within a 3D printed holder, the device employs electrokinetic manipulations to stack, separate, and desalt charged molecules on paper prior to spray into the MS. Due to counter-balanced electroosmotic flow and electrophoresis, charged analytes stack on the paper and desalting occurs in minutes. One end of the paper strip was cut into a […]
- Publication date: Available online 24 April 2024Source: Journal of ProteomicsAuthor(s): Christina E. Andronis, Silke Jacques, Francisco J. Lopez-Ruiz, Richard Lipscombe, Kar-Chun Tan
- Publication date: Available online 25 April 2024Source: International Journal of Mass SpectrometryAuthor(s): Małgorzata Kasperkowiak, Klaudia Antczak, Wojciech Jankowski, Marcin Hoffmann, Błażej Gierczyk, Rafał Frański