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- Design the electrocatalysts without noble metal is still a challenge for oxygen evolution reaction (OER) in acid media. Herein, we reported the manganese doping method to decrease the concentration of oxygen vacancy (Vo) and form the MnâO structure adjacent octahedral sites in spinel NiCo2O4âδ (NiMn1.5Co3O4âδ), which highly enhanced the activity and stability of spinel NiCo2O4âδ with a low overpotential (Ρ) of 280 mV at j = 10 mA cmâ2 and long-term stability of 80 h in acid media. The isotopic […]
- Eukaryotes produce a large number of cytochrome P450s that mediate the synthesis and degradation of diverse endogenous and exogenous metabolites. Yet, most of these P450s are uncharacterized and global tools to study these challenging, membrane-resident enzymes remain to be exploited. Here, we applied activity profiling of plant, mouse and fungal P450s with chemical probes that become reactive when oxidized by P450 enzymes. Identification by mass spectrometry revealed labeling of a wide range of active P450s, including six plant P450s, 40 […]
- Mascot Distiller ships with a number of reports, like box plot, ANOVA and volcano plot. We have a new tutorial for writing your own reports using Python and how to add them to Distiller's menus. #proteomics #MassSpec #MassSpectrometryhttps://www.matrixscience.com/blog/tutorial-creating-custom-reports-in-mascot-distiller.html
- Mass spectrometry (MS)-based data is commonly used in studying metabolism and natural products, but typically requires domain-specific skill and experience to analyze. Existing computational tools for non-targeted metabolite analysis (i.e., metabolomics) mostly rely on comparison to reference MS spectral libraries for metabolite identification, limiting the annotation of metabolites for which reference spectra do not exist. This is the case in plant secondary metabolites, where most spectral features remain unidentified. Here, we developed Spec2Class, a deep-learning algorithm for the identification and […]
- Extracellular vesicles (EVs) are now being increasingly recognized as an essential signalling entity in human plasma, linking them to health and various diseases. Still, their core protein and lipid componentry, which lie at the centre of EV form and function, remains poorly defined. Achieving this unmet milestone remains greatly hindered by abundant non-vesicular extracellular plasma components (non-EVs) in mass spectrometry-based analyses. Here, we performed high-resolution density gradient fractionation of over 80 human plasma samples to isolate circulating EVs, and systematically […]
- Mass-tolerant open search methods allow the high-throughput analysis of modified peptides by mass spectrometry. These techniques have paved the way to unbiased analysis of post-translational modifications (PTMs) in biological contexts, as well as of chemical modifications produced during the manipulation of protein samples. In this work, we have analyzed in-depth a wide variety of samples of different biological origin, including cells, extracellular vesicles, secretomes, centrosomes and tissue preparations, using Comet-ReCom, a recently improved version of the open search engine Comet-PTM. […]
- Abstract The past decades have marked the rise of metabolomics and lipidomics as the -omics sciences which reflect the most phenotypes in living systems. Mass spectrometryâbased approaches are acknowledged for both quantification and identification of molecular signatures, the latter relying primarily on fragmentation spectra interpretation. However, the high structural diversity of biological small molecules poses a considerable challenge in compound annotation. Feature-based molecular networking (FBMN) combined with database searches currently sets the gold standard for annotation of large datasets. Nevertheless, […]
- Publication date: Available online 17 March 2024Source: International Journal of Mass SpectrometryAuthor(s): Zhongmou Zhang, Yadan Wang, Yongjian Wang, Xianfu Wu, Shuangcheng Ma
- Publication date: Available online 16 March 2024Source: Journal of Chromatography BAuthor(s): Ryte Poskute, Praveen Kallamvalliillam Sankaran, Laura Sewell, Giordana Lepore, Rebecca Shrubsall, Lydia Dewis, Yasunori Watanabe, Vanessa Wong, Laura Pascual Fernandez, Rahul Mishra, Alexander Holt, Susie Sou, Claire Harris, Cristina Moreno Rodriguez, Ayca Cankorur-Cetinkaya, Jennifer Smith, Nikola Lonska, Adam Powell, Tingting Cui, Matthew Cheeks
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.4c00030
- The conversion of methane under ambient conditions has attracted significant attention. Although advancements have been made using active oxygen species from photo- and electro- chemical processes, challenges such as complex catalyst design, costly oxidants, and unwanted byproducts remain. This study exploits the concept of contact-electro-catalysis, initiating chemical reactions through charge exchange at a solid-liquid interface, to report a novel process for directly converting methane under ambient conditions. Utilizing the electrification of commercially available Fluorinated Ethylene Propylene (FEP) with water under […]
- Journal of Mass Spectrometry, Volume 59, Issue 4, April 2024.
- Journal of Mass Spectrometry, Volume 59, Issue 4, April 2024.
- Abstract Oral endocrine therapies (OET) for breast cancer treatment need to be taken over a long period of time and are associated with considerable side effects. Therefore, adherence to OET is an important issue and of high clinical significance for breast cancer patientsâ caregivers. We hypothesized that a new bioanalytical strategy based on liquid chromatography and high-resolution mass spectrometry might be suitable for unbiased adherence monitoring (AM) of OET. Four different biomatrices (plasma, urine, finger prick blood by volumetric absorptive […]
- A group of bacterial proteases, the Pro-Pro endopeptidases (PPEPs), possess the unique ability to hydrolyze proline-proline bonds in proteins. Since a protease its function is largely determined by its substrate specificity, methods that can extensively characterize substrate specificity are valuable tools for protease research. Previously, we achieved an in-depth characterization of PPEP prime-side specificity. However, PPEP specificity is also determined by the non-prime-side residues in the substrate. To gain a more complete insight into the determinants of PPEP specificity, we […]
- European Journal of Mass Spectrometry, Ahead of Print. Atmospheric pressure field desorption (APFD) mass spectrometry (MS) has recently been introduced as a new variant of field desorption (FD) mass spectrometry. The development aimed at providing the basic characteristics of FD-MS in combination with instruments equipped with an atmospheric pressure (AP) interface. Hitherto, APFD has been demonstrated to yield both positive and negative even electron ions of highly polar or ionic compounds as well as to enable the generation of positive […]
- Phytophagous insects are capable of detecting and locating suitable hosts, which emit volatile compounds. Polyphagous species appear to have a complex olfactory strategy given that their numerous hosts have diverse emission profiles. In particular, their hosts' volatile emissions share some of the same compounds, providing chemical bridges between them. However, the behavioural plasticity observed in insect host selection suggests that other volatiles have a complementary role. Here we explore how polyphagous insect specialization to detect and discriminate between hosts has […]
- Journal of Proteome ResearchDOI: 10.1021/acs.jproteome.3c00797
-  I need more time to spend on this, but I'm going to put it here anyway. I'm going to be petty for just a second and ask what club you have to be in to rename a method without the peer reviewers making fun of you? Before Josh Coon coined the phrase "parallel reaction monitoring" there was a TMS2 node on the Q Exactive. It sure as Albert Heck says PRM now. What's this one, though? Okay, so HLA peptides are super […]
- Abstract In this study, a novel quality control strategy was proposed, aiming to establish a multivariate specification for the processing step by exploring the correlation between colors, chemical components, and hemostatic effects of the carbonized Typhae pollen (CTP) using multivariate statistical analysis. The CTP samples were stir-fried at different durations. Afterward, the colorimeter and LCâMS techniques were applied to characterize the CTP samples, followed by the determination of bleeding time and clotting time using mice to evaluate their hemostatic effect. […]
-  I missed the 20TH MEETING OF THE US HUMAN PROTEOMICS ORGANIZATION thanks to my immune system being a slacker. The committee I chair, the US HUPO Virtual Media Outreach Committee (most of the group is above) sent me pictures like this. I just found this and I'm brazenly stealing it — Is this the same meeting that we'd been trying to interview all the invited speakers for? Yes. Well…not all of them, THE Proteomics Show has to fit into space around our […]
- Analytical ChemistryDOI: 10.1021/acs.analchem.3c05019
- PROTEOMICS, EarlyView.
- Publication date: Available online 14 March 2024Source: Analytica Chimica ActaAuthor(s): Luluil Maknun, Jitapa Sumranjit, Tuksadon Wutikhun, Ryszard Lobinski, Joanna Szpunar, Atitaya Siripinyanond